2 edition of Evidence of multipotency in immortalised cells derived from bone marrow stroma. found in the catalog.
Evidence of multipotency in immortalised cells derived from bone marrow stroma.
Andrew James Harrison
Thesis (Ph.D.), University of Manchester, Faculty of Medicine.
|Contributions||University of Manchester. Faculty of Medicine.|
|The Physical Object|
|Number of Pages||261|
The majority of T cells present in the bone marrow (BM) represent an activated/memory phenotype and most of these, if not all, are circulating T cells. Their lodging in the BM keeps them activated, turning the BM microenvironment into a “memory reservoir.” This article will focus on how T cell activation in the BM results in both direct and indirect effects on the hematopoiesis. W. S. Lee, Y. Suzuki, S. S. Graves et al., “Canine bone marrow-derived mesenchymal stromal cells suppress alloreactive lymphocyte proliferation in vitro but fail to enhance engraftment in canine bone marrow transplantation,” Biology of Blood and Marrow Transplantation, vol. .
cells in vari-ous animal models, using bone marrow-derived hematopoi-etic stem cells [26–31]. Moreover, marrow stroma-derived fibroblast-like, CD34 cells can give rise to CD34 cells with hematopoietic characteristics with regard to colony formation and long-term culture initiation , which re-cently was confirmed by others . Mesenchymal stromal cells (MSCs) are crucial elements in the bone marrow (BM) niche where they provide physical support and secrete soluble factors to control and maintain hematopoietic stem progenitor cells (HSPCs). Given their role in the BM niche and HSPC support, MSCs have been employed in the clinical setting to expand ex-vivo HSPCs, as well as to facilitate HSPC engraftment in .
Adipose-derived stem cells (ASCs) exhibiting mesenchymal stem cell (MSC) characteristics, have been extensively studied in recent years. Because they have been shown to differentiate into lineages such as osteogenic, chondrogenic, neurogenic or myogenic, the focus of most of the current research concerns either their potential to replace bone marrow as a readily available and abundant source. Normal bone marrow – generalities and function. The term ‘bone marrow’ (BM) refers to the tissue occupying the cavities under the cortex within the honeycomb of trabecular bone. Normal marrow is either red, consisting of the hematopoietic tissue, or yellow, composed mainly of fat cells .
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Bone marrow stromal cells (BMSCs) contains a subset of skeletal stem cells (SSCs) that are multipotent, capable of forming cartilage, bone, hematopoiesis-supportive stroma and marrow adipocytes. SSCs have been identified as pericytes, cells on the abluminal side of marrow sinusoids, and have demonstrated the ability to self-renew.
Bone marrow stromal cells are progenitors of skeletal tissue components such as bone, cartilage, the hematopoiesis-supporting stroma, and adipocytes. In addition, they may be experimentally induced to undergo unorthodox differentiation, possibly forming neural and myogenic by: Human bone marrow stroma stem cells, also known as mesenchymal stem cells (hMSC), have a demonstrative role in treating transient cerebral ischemia.
The routes for hMSC administration include intraperitoneal, intravenous, and intraarterial injections, among which intravenous is the most commonly by: 1.
Bone marrow. Bone marrow was the original source of MSCs, and still is the most frequently utilized. These bone marrow stem cells do not contribute to the formation of blood cells and so do not express the hematopoietic stem cell marker CD They are sometimes referred to as bone marrow stromal stem cells.
Cord cellsMeSH: D 1. Introduction. Although the presence of osteogenic cells in bone marrow has long been known (Friedenstein et al., ), the detailed nature of those cells has been described only presence of adherent fibroblast-like cells in bone marrow aspirates was also reported by Friedenstein et al.
().Since the culture contains cells which can generate single cell-derived colonies Cited by: Bone Marrow Derived Stem Cells. Bone marrow is a sponge-like tissue found within the bones.
It houses hematopoietic stem cells and other important growth factors needed for healing. Bone marrow derived stem cells have been used for treating various conditions for.
Abstract. We have developed protocols whereby a total of 30–90 × 10 6 hMSCs with an average viability greater than 90% can be produced in a single multilevel Cell Factory from a relatively small (1–3 mL) bone marrow aspirate in 14–20 d. It is possible to generate as many as 5 × 10 8 multipotent stromal cells (MSCs) from a single sample, depending on the number of Cell Factories seeded.
Hiroshi Tanaka and C. Tony Liang, Effect of platelet‐derived growth factor on DNA synthesis and gene expression in bone marrow stromal cells derived from adult and old rats, Journal of Cellular Physiology,2, (), ().
MSCs or MSC-like cells are not a unique feature of the bone marrow, as they also are found in tissues such as fat, umbilical cord blood, amniotic fluid, placenta, dental pulp, tendons, synovial membrane and skeletal muscle, though the complete equivalency of such populations has not been formally demonstrated using robust scientific methods (Rogers and Casper,Bieback and Kluter.
Collagenase treatment increased the number of obtainable EGFP-positive cells, suggesting a tight association of neural crest-derived cells with the bone marrow surface. Thus, the bone marrow appears to contain several distinct stem cell types of different developmental origins.
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Mesenchymal stem cells (MSCs) are bone marrow-derived cells that can differentiate into mesodermal cell lineages, and are easily isolated and propagated in vitro 5. MSCs can differentiate into a.
Mesenchymal stem cells (MSC), because of their multipotency and ease of purification and amplification, are an ideal stem cell source for cell therapies. Bone-marrow-derived stem cells (BMSC) can be used to develop MSC-like immortalized cell lines with large proliferation and differentiation potentialities.
Their immortalized status prevents the maintenance of MSC function and. Mesenchymal stem cells (MSCs) are multipotent cells that can be expanded and manipulated ex vivo.
These cells demonstrated three biological characteristics that qualify them for the use in cellular therapy: (1) potential of differentiation, (2) secretion of trophic factors and (3) immunoregulatory properties.
The bone marrow (BM) has been considered as the traditional source of MSCs and much. Multiple myeloma (MM) is a deadly hematological malignancy characterized by the uncontrolled growth and accumulation of monoclonal plasma cells in the bone marrow (BM), the presence of a monoclonal immunoglobulin fraction in the serum or urine, 1,2 renal failure, and osteolytic bone lesions.
3 MM cells depend on the BM microenvironment for their growth and survival through. Methods: Human bone marrow-derived mesenchymal stromal/stem cells were encapsulated in tyramine-modified hyaluronic acid (HA-Tyr) hydrogels, with crosslinking initiated by the addition of.
Mesenchymal stem cells can be isolated from bone marrow stroma and other sources, and the transient amplifying pool derived from it can be differentiated towards various mesenchymal pathways, for example, osteogenesis, chondrogenesis, adipogenesis and others. Introduction. Bone marrow stromal cells (BMSCs, also called bone-marrow-derived mesenchymal stromal cells) are heterogeneous populations that likely contain varying levels of tripotent (osteogenic, adipogenic, and chondrogenic [OAC]) stem-cell-like cells; cells with restricted potency (bi- uni- and nullipotent), committed precursors, and other stromal cell types.
Roshanak Ghazanfari, Hongzhe Li, Dimitra Zacharaki, Hooi Ching Lim, Stefan Scheding, Human Non-Hematopoietic CD pos /CDa low/neg Bone Marrow Stroma Cells Fulfill Stringent Stem Cell Criteria in Serial Transplantations, Stem Cells and Development, /scd, 25, 21, (), ().
Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. Research has demonstrated the potential of multipotent adult progenitor cells for treating ischemic injury and cardiovascular repair; however, understanding of multipotent adult progenitor cells in orthopedic applications remains limited.
Metformin, a popular drug used to treat diabetes, has recently gained attention as a potentially useful therapeutic agent for treating cancer. In our research metformin was added to in vitro cultures of bone marrow-derived multipotent mesenchymal stromal cells (BMSCs) and Balb/3T3 fibroblast at concentration of 1 mM, 5 mM, and 10mM.Mesenchymal stromal cells (MSCs) are nonhematopoietic, multipotent progenitor cells that differentiate into bone marrow (BM) stroma as well as adipocytes, chondrocytes, and osteocytes.
Initially expanded from BM, MSCs can also be culture-expanded from other sources, including umbilical blood, adipose tissue, and dental pulp. Bone marrow is the tissue comprising the center and the epiphysis of bones, which is the place where new blood cells are produced.
Bone marrow has .